Journal: Oncology Letters

Article Title: Increased soluble E‑cadherin of spheroid formation supplemented with fetal bovine serum in colorectal cancer cells

doi: 10.3892/ol.2023.13793

Figure Lengend Snippet: Densitometric results of western blotting on SNU-C5 and SNU-C5/5-FUR cells.

Article Snippet: CREB (1:1,000; cat. no. CSB-PA005947HA01HU; Cusabio Technology, LLC), fibronectin (1:2,000; cat. no. CL54951AP; Cedarlane Laboratories), p21 (1:1,000; cat. no. 60214-1; Proteintech Group, Inc.) and p90RSK (Ab348; 1:1,000; cat. no. 79-554; Prosci, Inc.) were obtained from the corresponding listed company.

Techniques: Western Blot, Marker

Journal: Aging (Albany NY)

Article Title: Activation of α7 nAChR by PNU-282987 improves synaptic and cognitive functions through restoring the expression of synaptic-associated proteins and the CaM-CaMKII-CREB signaling pathway

doi: 10.18632/aging.102640

Figure Lengend Snippet: Activation of α7 nAChR activates the calcium signaling pathway in Aβ oligomer and KN93-treated neurons. Protein levels in each group were determined by western blot analysis and β-actin was used as an internal control. The x-axis indicates the Control, hippocampus cells of the WT rat; Control + KN93, the WT hippocampus cells treated with CaMKII inhibitor (KN93); Control + Aβ, the WT hippocampus cells treated with Aβ; Control + PNU, the WT hippocampus cells treated with PNU; PNU + Aβ, the WT hippocampus cells treated with PNU and Aβ; KN93+PNU+Aβ, the WT hippocampus cells treated with KN93, PNU and Aβ; KN93+PNU, the WT hippocampus cells treated with KN93 and PNU; KN93+Aβ, the control group treated with KN93 and Aβ. The Y-axis indicates the protein expression levels as a percentage of the control. The results demonstrated that the protein expression of CaMKIIα was significantly decreased in KN93 or Aβ oligomer-treated neurons, whereas expression of CaMKIIα was increased in the PNU groups (PNU+Aβ, KN93+PNU+Aβ and KN93+PNU). This result indicated that α7 nAChR activated the CaM-CaMKII-CREB signaling pathway. Data are presented as the mean ± standard deviation. * P<0.05, ** P<0.01 vs. control; # P<0.05, ## P<0.01 vs. Control+Aβ, △△ P<0.01 vs. Control+KN93.

Article Snippet: Rabbit anti-PSD95 polyclonal antibody (cat. no. GTX133091), rabbit anti-SNAP25 polyclonal antibody (cat. no. GTX113839), rabbit anti-DYN1 polyclonal antibody (cat. no. GTX110379), rabbit anti-CREB monoclonal antibody (cat. no. GTX61140) and rabbit anti-β-actin polyclonal antibody (cat. no. GTX109639) were purchased from GeneTex, Inc. (Irvine, CA, USA).

Techniques: Activation Assay, Western Blot, Expressing, Standard Deviation

Journal: Aging (Albany NY)

Article Title: Activation of α7 nAChR by PNU-282987 improves synaptic and cognitive functions through restoring the expression of synaptic-associated proteins and the CaM-CaMKII-CREB signaling pathway

doi: 10.18632/aging.102640

Figure Lengend Snippet: Activation of α7 nAChR by PNU-282987 improves synaptic and cognitive functions through restoring the expression of synaptic-associated proteins and the CaM-CaMKII-CREB signaling pathway. Synapses are the basic structure for the connection between neurons, and the function of a synapse is largely associated with synaptic-associated proteins. The present study found that activation of α7 nAChRs by PNU-282987 could largely restore the expression level of synaptic-associated proteins, including SYN, PSD95, SNAP25, DYN1 and AP180, which are downregulated by deposition of Aβ. α7 nAChRs belong to the ligand-gated ion channel coupled receptors, and form an ion channel in the center, which mainly regulates the flow of Ca 2+ plasma inside and outside the cell. As a second cytoplasmic messenger, Ca 2+ can flow into the cells through α7 nAChRs and bind to CaM to form an active CaM complex. The CaM complex can bind to CaMKII, which changes the CaMKII spatial conformation and phosphorylates CaMKII protein. Phosphorylated CaMKII enters the nucleus to catalyze CREB phosphorylation. CREB is located downstream of the α7 nAChR signal transduction pathway and acts as a transcription factor that regulates the growth and development of neurons and the formation of long-term memory.

Article Snippet: Rabbit anti-PSD95 polyclonal antibody (cat. no. GTX133091), rabbit anti-SNAP25 polyclonal antibody (cat. no. GTX113839), rabbit anti-DYN1 polyclonal antibody (cat. no. GTX110379), rabbit anti-CREB monoclonal antibody (cat. no. GTX61140) and rabbit anti-β-actin polyclonal antibody (cat. no. GTX109639) were purchased from GeneTex, Inc. (Irvine, CA, USA).

Techniques: Activation Assay, Expressing, Transduction

Journal: Oncotarget

Article Title: Long-term neurocognitive dysfunction in offspring via NGF/ ERK/CREB signaling pathway caused by ketamine exposure during the second trimester of pregnancy in rats

doi: 10.18632/oncotarget.16042

Figure Lengend Snippet: Ketamine administered during the second trimester perturbed NGF and down-regulated ERK, p-ERK, MSK, CREB, p-CREB, SYP, SYN, and PSD95 in the hippocampi of offspring. On postnatal day 30, after behavioral tests, brains were harvested for western blot analyses to determine the expression of NGF, ERK, p-ERK, MSK, CREB, p-CREB, SYP, SYN, and PSD95. (A–I ) Above are representative immunoblots for the expression levels of the corresponding proteins, and below are the histograms of the quantification of the corresponding proteins normalized to GAPDH. Data are expressed as mean percentage ± SEM of control mean values. * p < 0.05.

Article Snippet: The separated proteins were transferred onto nitrocellulose (NC) membranes and incubated with rabbit anti- GAPDH, NGF, ERK, p-ERK, CREB, p-CREB, MSK1, SYP, SYN, and PSD95 (1:600, EnoGene, Nanjing, China) overnight at a temperature of 4 °C.

Techniques: Western Blot, Expressing, Control

Journal: Oncotarget

Article Title: Long-term neurocognitive dysfunction in offspring via NGF/ ERK/CREB signaling pathway caused by ketamine exposure during the second trimester of pregnancy in rats

doi: 10.18632/oncotarget.16042

Figure Lengend Snippet: Ketamine administered during the second trimester down-regulated ERK, p-ERK, MSK, CREB, p-CREB, SYP, SYN, and PSD95 in PC12 cells during in vitro experiments. SCH772984 is the inhibitor of the ERK protein. Through the contrast between S group and C group, a very good effect of the inhibitor is revealed. (A) Representative immunoblots for expression levels of the corresponding proteins. (B–I ) Histograms that quantify the corresponding proteins normalized to GAPDH, in which the data are expressed as mean percentages ± SEM of control mean values. * p < 0.05

Article Snippet: The separated proteins were transferred onto nitrocellulose (NC) membranes and incubated with rabbit anti- GAPDH, NGF, ERK, p-ERK, CREB, p-CREB, MSK1, SYP, SYN, and PSD95 (1:600, EnoGene, Nanjing, China) overnight at a temperature of 4 °C.

Techniques: In Vitro, Western Blot, Expressing, Control